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Genotyping by Sequencing
Experts

Rob Elshire is the lead developer of the original GBS method.
We specialise in delivering high quality, consistent GBS data sets to collaborators around the world.

Contact Us About us
PLOS One Open Access Peer Reviewed Research Article

7244

GBS Paper Citations

103

Species Analysed

Flag of New Zealand

25

Countries

World Map Shades of Blue

6

Continents

What our collaborators say about our genotyping-by-sequencing service.

Working with the Elshire Group has been an absolute joy.
Stephanie
Woman with bird.
I particularly like working with the Elshire Group because of the ease of communication, their flexibility to address specific client needs and their reliability in respect to high quality data output and analysis.
Tobias
man in rice field
Working with the Elshire Group was a delightful experience - their clear instructions, caring nature, and passion for the field turned a daunting task into a breeze; their commitment to exceptional results left a lasting impression. Looking forward to our next venture together
Ange
Ange
I really appreciated the guidance and communication I received from day one, especially when we consider how I had never worked with any Rad-seq process before.
Nimeshika
Nimeshika
Thank you for convincing us that we’ve made the right decision by outsourcing this job to you. Your service delivery was top notch and we wouldn’t mind coming again, and again.
Kanyesigye
Kanyesigye
Working with The Elshire Group provided us with a gold mine of high-quality GBS information at a reasonable price. The team was helpful and insightful throughout the process, from quoting, QC of DNA extraction, delivery and downstream data analysis.
Itay
Itay
Their patience and involvement were key to the success of this first-of-a-kind genetic study in Peru.
Antoine
Antoine
The Elshire Group takes the guesswork out of the genomics process, which lets me focus on the myriad other aspects of my project.
Leah
Minnow in a hand
I work with The Elshire Group on a couple of GBS projects in sesame. From the beginning of our project until the delivery of the high-quality results, the escort was absolutely professional along with kindness and flexibility
Idan
Idan
Rob and Robyn were excellent to work with. They clearly communicated with us throughout each step of the process and were committed to generating very high-quality genetic data for us. As a PhD student who was simultaneously learning about genomics while preparing my samples for sequencing, they could not have been more supportive or helpful during this process.
Sophie
Sophie
Working with the Elshire Group was truly remarkable, thanks to their genuine care and close collaboration with clients to provide exceptional genotyping by sequencing data. This resulted in a professional and personable experience that exceeded expectations.
María
María
The Elshire group has done a great job for us. I particularly appreciate how they have worked with us through the QC process, and effectively lowered the technology barrier for us. A great option for first time users of their technology.
David
David
We studied the Pisum genus, which includes two species fairly genetically different from each other. Thus we had our concern about the number of polymorphic sites. The result was a high number of genotype calls with good genome coverage. The Elshire Group has been very approachable and provided helpful advice throughout the DNA extraction and quality control steps, ensuring we would get high-quality calls. We were pleased with choosing them as a GBS service provider.
Ron
Ron
Superior support and service provided with Kiwi quickness! We have really valued our collaborations with the Elshire group.
Jake
Jake
Great competence, professionalism, courtesy and availability have been the basis of this fruitful collaboration for years.
Francesca
Francesca
I really enjoyed working with the Elshire Group, particularly because of the transparency and honesty they had at each step of my Genotyping by Sequencing project. Their knowledge and assistance throughout the whole project were extremely useful for someone new to the genomic space.
Ben
Ben
In 2017 I started to work with the Elshire Group, and we realized the first GBS in olive. It was a great experience! For me was the first approach to NGS world. Thanks Rob for your competence and friendship
Cinzia
Cinzia
The Elshire group have been amazing to work with thanks to their supportive and inclusive attitude towards researchers (and humans in general) of all backgrounds - for instance kiwi-catching forest-ecologists like myself!
Malin
Malin

A selection of species we have worked with.

plant breeding, SNP Genotyping, Genotyping Services,Single Nucleotide Polymorphism (SNP) Analysis
Capsicum anuum

Capsicum

Horticulture Breeding

Next-Generation Sequencing (NGS), GBS Service, DNA Sequence Variation
Zea mays

Maize

Agriculture Breeding

Domestic chickens, Variant Calling, Genomic Profiling
Gallus gallus

Chicken

Animal Breeding

SNP Discovery, High-Quality Genotyping
Impatiens capensis

Orange Jewelweed

Plant Ecology

Single Nucleotide Polymorphism (SNP) Analysis
Thinornis novaeseelandiae

Tūturuatu / Shore Plover

Avian Conservation Biology

Mammalian Genotyping, Easy to use, Cost Effective
Procavia capensis

Rock Hyrax

Mammalian Ecology

Genomic Data Analysis, Population Genetics Studies, Genetic Testing Services
Carum carvi

Caraway

Orphan Crop Breeding

Insect genomics, SNP Discovery, Genomic Research Service
Tipulidae spp.

Cranefly

Insect Ecology

Aquatic Diversite, Mollusc, DNA Sequence Variation, Genotyping by Sequencing
Tridacna maxima

Cook Island Clam

Molluscan Ecology

What is Genotyping by Sequencing?

Genotyping-by-Sequencing (GBS) is a method for molecular marker discovery and genotyping which is widely used in many areas of genomics research. Genotyping-by-sequencing (GBS) allows the simultaneous discovery and genotyping of thousands to millions of single nucleotide polymorphisms depending on the genetic diversity sampled. It was first developed to provide a low cost, high throughput genetic marker platform for plant breeding in corn by Rob Elshire and his colleagues at Cornell University.

That was just the beginning. In addition to agricultural applications, genotyping-by-sequencing (GBS) has enabled the use of modern genomics tools for researchers in fields such as ecology and conservation biology. This system can be used without a reference genome and is ideal for non-model organisms. Working with Rob Elshire, the original developer, on your genotyping-by-sequencing (GBS) project is easy, go to our services page to find out more and our contact page to get the process started.

How does it work?

Genotyping-by-sequencing (GBS) combines the data generation power of modern DNA sequencing with long used and well known molecular biology techniques in a streamlined method to generate many molecular markers. To take advantage of the huge amount of sequence data generated by modern sequencing and reduce the cost per sample, genotyping-by-sequencing (GBS) uses an efficient DNA bar coding system which allows us to combine many samples for sequencing.

5 Main Steps

  1. Cut up the DNA into fragments in a predictable way using methylation sensitive restriction enzymes. One can think of these as very specialised molecular scissors.

  2. Attach specially designed DNA molecules (incorporating bar codes) to the cut DNA using DNA ligase. Ligase can be thought of as glue or sellotape.

  3. Make many, many copies of the DNA molecules using a DNA polymerase to make a library — much like a copy machine.

  4. Sequence the library thereby generating a large data set.

  5. Perform an analysis of the data comparing the sequences and looking for differences. These differences are genetic markers

Ready to plan your genotyping-by-sequencing experiment with us?

We are always happy to help.
Contact Us